DBiT-seq platform for high-resolution spatial epigenomics

Map open chromatin and chromatin marks directly on tissue, connect accessibility with morphology, and uncover regulatory programs — on the same slide you already know and trust.

In situ chemistry
Label biomolecules within intact sections using on-slide enzymatic reactions — no dissociation.
Spatial barcoding
Create a coordinate grid with two orthogonal microfluidic chips; each pixel receives unique A × B barcodes.
Lysis & library prep
Extract barcoded molecules, build indexed libraries, and generate pixel-level maps after sequencing.
Protocol Overview
DBiT-seq workflow
DBiT-seq and in-situ chemistry workflow diagram: label, hybridize and ligate spatial barcodes, image, then lyse and sequence
Label · Hybridize barcodes (X) · Ligate barcodes (Y) · Image · Sequence
Microfluidics
Spatial barcoding chip
Microfluidic chip used to deliver orthogonal spatial barcodes across the tissue section
48,400 pixels · 10 × 10 µm
Instrumentation
Runs on standard equipment
Benchtop instrumentation for the DBiT-seq spatial epigenomics workflow
No custom instruments required
10 × 10 µm pixels 48,400 spots / chip 5.5 × 5.5 mm chip 1 × 3 slide