Our Platform

Bringing transformative spatial-omics to researchers with microfludics and Next Generation Sequencing

Our Platform DBiT-Seq, can be broken down into three Major Steps

In Situ Chemistry

Spatial Barcoding

Lysis

In situ Chemistry

Biomolecules of interest within the tissue are labeled with a known molecular barcode by adding enzymes, molecular probes, or antibodies through a light permeabilization technique.

Illustration of a laboratory experiment involving a liquid being applied to a surface with bacteria, and a pink petri dish containing scattered bacteria, with a pattern of sperm cells on a pink background.

Spatial Barcoding

We create a coordinate system within the tissue using two microfluidics chips. Each intersection is a square-shaped element, a pixel, with biomolecules uniquely labeled with Barcode A and Barcode B pairs at a known location

Diagram of individual fluorescent microscopy slides on a tray and a corresponding cross-sectional view showing different fluorescent signals in labeled regions A1, A2, B1, and B2.

Lysis

The tissue is lysed, and our array of pixels mapping the cellular microenvironment is extracted for library preparation. The library is sequenced and processed using standard single-cell bioinformatics pipelines.

Abstract digital equalizer graphic with colorful squares on dark background.
Abstract geometric artwork with overlapping rectangles and squares in shades of blue, purple, and white on a black background.

The Platform has enabled higher performance spatial mapping

Comparison of mouse cerebellum images across three generations, Gen 1, Gen 2, and Gen 3, showing differences in microscopy techniques and image details, with a table detailing the platforms, spot size, number of spots, center-to-center distance, and field of view for each generation.

Check out our video for more