Spatial CUT&Tag: High-Resolution Chromatin Profiling with Spatial Context​

Introduction

Spatial CUT&Tag enables high-resolution mapping of histone modifications and chromatin-associated proteins directly within intact tissue, preserving native architecture while revealing regulatory patterns that bulk methods cannot capture. Developed in the Fan Lab and published in Science, the method delivers reproducible, low-background chromatin profiles with strong concordance to ENCODE ChIP-seq. This white paper highlights how Spatial CUT&Tag uncovers spatially distinct regulatory states—such as tumor-specific chromatin features and localized enhancer activity—to provide deeper insight into tissue heterogeneity and disease biology.