Turn intact tissue into a high-resolution spatial epigenomic map.

Profile chromatin accessibility, histone modifications, RNA, and DNA methylation directly on tissue — without dissociation or specialized instrumentation.

The basics

Two names, one platform: DBiT-seq is the published spatial barcoding method, and FlowGel is how AtlasXomics packages that same core chemistry for your lab.

DBiT-seq Deterministic Barcoding in Tissue sequencing

The spatial barcoding methodology behind the platform — mapping molecules to their location in intact tissue. It's the approach established and cited across peer-reviewed publications.

Published method
FlowGel

The AtlasXomics pre-assembled consumable built on that same core chemistry — delivering the barcoding without the traditional microfluidic setup, so the workflow runs on standard lab equipment.

AtlasXomics product
How it works

From tissue to spatial data

Five steps take an intact section to a pixel-level epigenomic map — mount, label in situ, encode spatial position, lyse and recover, then sequence and reconstruct.

Protocol Overview
Tissue-to-data workflow
5 steps · standard NGS
How it works: from tissue to spatial data — five-step DBiT-seq workflow
Spatial barcoding

How X × Y barcoding works

Two orthogonal barcode patterns flow across the tissue. Their intersections label every molecule with a unique X,Y coordinate (e.g., X2Y3) that survives into sequencing and is used to place each read back in its original location.

Coordinate System
X × Y spatial barcoding
Xn × Ym → unique coordinate
How X by Y spatial barcoding works — orthogonal barcodes create unique coordinates carried into sequencing

One spatial barcoding platform. Multiple biological readouts.

Spatial ATAC-seq
Chromatin accessibility
Which regulatory programs are active?
Learn more →
Spatial CUT&Tag
Histone marks / proteins
Where are specific chromatin states located?
Learn more →
Spatial CoProfiling
ATAC + RNA
How do regulatory state and expression relate?
Learn more →
Spatial ATAC-TAPS+
Accessibility + methylation
How do chromatin accessibility and DNA methylation interact?
Learn more →

FlowGel simplifies spatial barcoding

AtlasXomics FlowGel spatial barcoding consumable on a standard slide
FlowGel spatial barcoding workflow detail
Pre-assembled spatial barcoding
Eliminates the more cumbersome microfluidic preparation associated with traditional DBiT-seq.
Consistent 10 µm grid
48,400 spatial locations across a 5.5 × 5.5 mm area for high-resolution coverage.
Simple workflow
Designed to integrate with standard histology slides and laboratory equipment.
One platform across assays
The same spatial barcoding workflow supports ATAC, CUT&Tag, co-profiling, and methylation assays.

What you need

Run FlowGel with standard slides, a fluorescence microscope, routine molecular biology equipment, and NGS access — we supply the barcoding consumables, reagents, protocols, and analysis support.

Choose how you want to work

Send us tissue for full white-glove service, or run spatial epigenomics in your own lab with FlowGel kits, protocols, and support.

Proven and published

DBiT-seq and FlowGel are described in peer-reviewed publications across leading journals.

Ready to explore your project?
Our scientists are here to help you design the right assay and answer your questions.