AGBT 2026 — New Research

Integrating Spatial Open Chromatin & DNA Methylation Profiling with Spatial ATAC-TAPS+

Co-registered chromatin accessibility and DNA methylation mapping in a single tissue section — resolving epigenetic heterogeneity that bulk methods miss.

Spatial ATAC-TAPS+ poster presented at AGBT 2026
Click to view full poster
27,923
Clustered nuclei at 10 µm resolution
~8,226
Median fragments per spot
8
Distinct epigenetic compartments resolved
2,195
Differentially methylated genes identified

Key Findings

Dual-Modality in One Section

TAPS+ conversion preserves spatial ATAC library integrity — fragment size distribution, FRiP, and TSS enrichment remain stable before and after conversion.

Spatial Regulatory Compartments

Unsupervised clustering identifies eight spatially coherent epigenetic compartments with coordinated accessibility–methylation divergence across tumor regions.

Interpretable Chromatin States

Integrated accessibility and 5mC define four functional chromatin states — active, poised, repressed, and latent — enabling direct biological interpretation.

Spatial ATAC-TAPS+ Workflow

Step 1
Tn5 In Situ Tagmentation
Step 2
Microfluidic Spatial Barcoding
Step 3
Tissue Imaging & Co-Registration
Step 4
TAPS+ Conversion (5mC/5hmC)
Step 5
Library Prep & Sequencing
Step 6
Dual-Modality Spatial Maps