Spatial CUT&Tag
Defining cell types with spatial CUT&Tag
Spatial CUT&Tag
Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level
Yanxiang Deng¹ ², Marek Bartosovic³, Petra Kukanja³, Di Zhang¹, Yang Liu¹ ², Graham Su¹ ², Archibald Enninful¹ ², Zhiliang Bai¹, Gonçalo Castelo-Branco³ ⁴, Rong Fan¹ ² ⁵
Spatial CUT&Tag protocol
Target chromatin modifications to map gene regulation
Anibody validation for sensitivity and specificity
Targets
Validated Targets
Common Post-Translational Modifications (PTMs)
Transcriptional Regulator - CTCF
Transcription Machinery - RNA Pol II
Targets in development
Histone Modification Writers/Readers - BRD4 and EZH2
Hormone Receptors - Estrogen Receptor (ER) and Androgen Receptor (AR)
Validate performance in bulk assay
Validate specificity in tissue
